ABSTRACT
Objective To compare the applied signification of optical and ultrasonic biometry in observing the axial length of eyes with vitreous hemorrhage and retinal detachment. Methods This is a prospective clinical study. We randomly selected 57 cases (57 eyes) with vitreous hemorrhage and (or) retinal detachment from May 1, 2016 to May 31. Patients had not been performed eye surgery, also denied the history of ocular trauma and excluded other eye diseases. 25 eyes with vitreous hemorrhage, 24 eyes with retinal detachment, 8 eyes complicated with vitreous and retinal detachment hemorrhage. At 24 hours before surgery, we measured axial length using the Lenstar LS 900, immersion B-ultrasound (IB) and immersion A-ultrasound (IA). The differences between three methods were assessed with paired sample t-test. Results IB and IA data was collected from all 57 eyes, there was no statistical significance between measurements of IB and IA (P > 0.05). In the eyes with serious refractive medium opacity (36 eyes), the results showed no statistical significance difference between measurements of IB and IA (P > 0.05). The measurements of three groups can be obtained from the rest eyes (21 eyes): significant statistical difference of the measurements between IB and Lenstar was detected[(24.057±1.441)mm vs(23.470±1.775)mm],(t=3.099, P < 0.05) same results between IA and Lenstar was obtained [(23.962±1.443) mm vs (23.470±1.775) mm], difference was statistically significant (t=2.643, P < 0.05). But the measurements showed no statistical significance difference between the measurements of IB and IA (P > 0.05). Conclusions In the eyes with vitreous hemorrhage and (or) retinal detachment, the measurements of axial length could not be achieved or existed deviations on account of refractive medium opacity when using optical biometry. Under such circumstances, we should choose IA or IB as the optimization method to obtain measurements, in order to get much more accurate results.
ABSTRACT
Objective To investigate the expression and change of neuroglobin (NGB) gene on hypoxic-ischemic brain injury. Methods Fourty-eight wistar rats of 7days old were divided into 6 groups randomly. Five study groups, study (1 min), study (5 min), study (15 min), study (30 min), study (60 min), and control group. The brain tissues were taken at 1 min, 5 min, 15 min, 30 min and 60 min after brain hypoxic-ischemia injury, and nucleic and immunohistochemistry method were used. One-way method of Stata soft-bage was used for statistics. Results There were time-depended pattern of the expression of NGB gene. It increased rapidly at 1 min after ischemia (A:1.236), there was significant difference from control group (A:0.641; P
ABSTRACT
Objective In order to explore the localization of NGB mRNA in adult rat brain. Methods In the present study,the localization of NGB mRNA in brain of the adult rat was examined by in situ hybridization histochemistry using digoxigenin labelled cRNA probes. Results Transcripts of NGB mRNA were showed to be widely distributed throughout the adult rat brain,including cerebral cortex,hippocampus,thalamus,hypothalamus,cerebellum and olfactory bulb.Conclusion\ Our result suggested that NGB gene might play an important role in the central nervous system,possibly related to the oxygen supply of the neuron.